The best Side of attune flow cytometer
The best Side of attune flow cytometer
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Knowledge-enriched characterization of cell populations. Morphological data from illustrations or photos can increase for the richness of flow cytometry knowledge. As an example, the figure shows an otherwise regular apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in each population to expose morphologically distinctive attributes. These insights could not are actually obtained from multiplex staining on your own.
As samples are obtained around the Attune CytPix Flow Cytometer, the higher-velocity brightfield digicam captures and outlets pictures of detected events in a charge as much as 6,000 visuals per second, according to the flow fee and impression measurement. For better overall flexibility, Attune Cytometric Program permits you to adjust the graphic capture frequency as wanted and choose a specific gate for impression capture, if essential.
(B) RAW 264.7 macrophages in many cases are annotated inaccurately with respect to particle depend due to their complexity and noncircular borders. The amount of singlets accessible for Examination improves after the consumer trains and employs a more accurate design.
Explore new insights into your experimental data by visualizing populations employing merged fluorescence and morphology details. Our software program interface wizards allow downsampling, concatenation (merged data files) and dimensionality applications that happen to be built into your software program for an intuitive, streamlined info Examination knowledge.
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On top of that, it is actually illuminating to find out how many doublets slip through regular singlet gates, and great to validate the position of unforeseen double-favourable gatherings in my Investigation."
Optimizing gating technique. Even sturdy manual singlet gating is error-vulnerable and remains a subjective conclusion position in Pretty much all flow cytometry assays. Imaging may be used to confirm and adjust gates to incorporate only solitary cells of interest.
Our researchers will design and style panels for you personally as many times you will need—totally free, no product invest in essential.
Early indications of this contamination experienced at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also enables you to use morphological capabilities to find out appealing subpopulations that could not be evident from flow cytometry information by itself.
Consumers can view event visuals in serious-time to confirm sample high quality, optimize protocols, and report the morphology of every cell inhabitants determined for Evaluation. Imaging capture also benefits from acoustic focusing, which really helps to placement the cells in order that a pointy, centered impression is attained.
AI-pushed automatic graphic Examination While using the Attune Cytometric Software interprets party capabilities into distinctive morphology parameters which can be combined with typical fluorescence and scatter parameters to reveal remarkably reproducible inhabitants changes and distinctive subsets.
Application note: Automated graphic Evaluation lessens user-to-user variability in flow cytometry gating techniques
Leverage the power of State-of-the-art engineering and innovation to accelerate your discoveries. Trust in the Attune Flow Cytometer family members to deliver the reliability, speed, and accuracy that established your do the job apart.
No wash/no lyse leukocyte Examination with imaging. Cells were being acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Specialist UX/UI enables buyers to practice AI versions for expanded apps. (A) The “Coach Model” feature gives annotation applications and real-time product training precision.
At the heart of each and every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which makes use of seem waves to meticulously align cells within the flow cell. This innovative solution surpasses traditional hydrodynamic approaches, causing more quickly processing speeds, Extraordinary data excellent, and enhanced sample throughput.
Since the mobile line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the photographs and were being in the position to document the microbial infection at the very check here least five days previously.
The Attune CytPix Flow Cytometer makes it possible for users to visualise samples during acquisition, verify the precision of gating techniques, and aid success with trusted documentation for publication reasons.
What appeared to be a homogeneous populace is in fact more diverse—an Perception that needs to be regarded when interpreting benefits.